Researchers at the University of Toronto (U of T) have uncovered new insights on the genetic causes of amyotrophic lateral sclerosis (ALS). These findings could uncover a new way to detect a genetic predisposition to amyotrophic lateral sclerosis before the disease strikes.
A common mutation associated with amyotrophic lateral sclerosis is an unstable repeated DNA sequence within the C9orf72 gene that could reach into the thousands. However, it is unknown how many repeats would be sufficient to cause the disease. A way to predict if the number of repeats increases to the damaging range is the addition of methyl groups to the repeats. This modifies the DNA, shutting off production of C9orf72.
The study, led by Professors Ekaterina Rogaeva and Lorne Zinman of the U of T Division of Neurology, followed 3 generations of a single family. Although the grandparents and parents in the family were healthy, 4 of 5 adult children possessed the repeat methylation, as a result of a damaging mutation in the C9orf72 gene responsible for amyotrophic lateral sclerosis. Two of them have already died due to complications of amyotrophic lateral sclerosis.
The cause of amyotrophic lateral sclerosis is unknown in about 90% of patients. The remaining 10% are attributed to genes passed down through parents. Scientists have long understood that people with genetically acquired amyotrophic lateral sclerosis have a parent with the disease. However, the study by the Rogaeva and Zinman team found that neither parent nor grandparents had amyotrophic lateral sclerosis.
In most people, it's common for the C9orf72 gene to have less than 30 repeats. The father examined in this study had 70 unmethylated repeats, which is not sufficient to cause amyotrophic lateral sclerosis. Yet, in his children the mutation expanded to more than 1000 repeats. Although the father had an overexpression of C9orf72, the children had reduced expression of that gene. The researchers believe these factors explain how his children acquired amyotrophic lateral sclerosis, while he remained unaffected.
"With further research, we may be able to use this information to develop a genetic test that can identify if parents are likely to pass a mutation that may cause ALS in their offspring," said Zinman, who is also an associate scientist at Sunnybrook Research Institute and a staff neurologist at Sunnybrook Health Sciences Centre.
By expanding this research to include other multigenerational families, as well as study unaffected parents, the team hopes to identify a range of C9orf72 repeats that can be used to predict a genetic predisposition to ALS in children.
"We were fortunate to have the opportunity to look at a multiple generation family," said Rogaeva, who is also a professor at the Tanz Centre for Research in Neurodegenerative Diseases. "It gave us a much more complete picture of the genetic development between each branch of the family tree."
Their research was published in June 4 issue of The American Journal of Human Genetics.
Source: News Release
University of Toronto
June 3, 2015