New model of amyotrophic lateral sclerosis is based on human cells from autopsied tissue

Aug 11, 2011

By isolating cells from patients' spinal tissue within a few days after death, researchers funded by the National Institutes of Health have developed a new model of amyotrophic lateral sclerosis (ALS). They found that during the disease, cells called astrocytes become toxic to nerve cells – a result previously found in animal models but not in humans. The new model could be used to investigate many more questions about amyotrophic lateral sclerosis, also known as Lou Gehrig disease.

Amyotrophic lateral sclerosis can run in families, but in the majority of cases, it is sporadic, with no known cause. The researchers derived astrocytes from patients who had succumbed to either type of amyotrophic lateral sclerosis, and found that the cells secrete toxic factors that cause nerve cells to degenerate. A similar mechanism has been found in mouse models of amyotrophic lateral sclerosis.

"The mouse models capture a type of familial ALS that accounts for only 2% of all cases. The field has begged for new disease models that can provide a clear window into sporadic ALS," said senior author Brian Kaspar PhD, an investigator at the Nationwide Children's Research Institute (NCRI) in Columbus, Ohio. His collaborators were Jerry Mendell MD, director of NCRI's Center for Gene Therapy, and Arthur Burghes PhD, a professor of molecular and cellular biochemistry at Ohio State University in Columbus. Drs Kaspar and Mendell also hold faculty positions at Ohio State.

The research is reported in Nature Biotechnology and was funded in part by NIH's National Institute of Neurological Disorders and Stroke (NINDS), including a $1.7 million stimulus grant made possible by the American Recovery and Reinvestment Act.

Amyotrophic lateral sclerosis is characterized by the death of motor neurons, which are muscle-controlling nerve cells in the spinal cord. As these neurons die, the body's voluntary muscles weaken and waste away. Death within 5 years of diagnosis is common. The only approved treatment, riluzole, extends life expectancy by just a few months.

About 90% of amyotrophic lateral sclerosis cases are sporadic and 10% run in families. Mutations in the superoxide dismutase1 (SOD1) gene are found in about one fifth of people with familial amyotrophic lateral sclerosis, and for decades, experts have theorized that the gene holds clues to sporadic amyotrophic lateral sclerosis. Laboratory mice carrying human SOD1 mutations develop signs of amyotrophic lateral sclerosis as they age, and have been widely used to investigate the causes and potential treatments for the disease.

Recent NIH-funded studies showed that astrocytes derived from these SOD1 mice are toxic to motor neurons. Astrocytes – lesser known than neurons and usually described as playing a supportive role –became prime suspects for killing neurons in amyotrophic lateral sclerosis.

At the same time, however, researchers have questioned whether SOD1 mice are useful– and whether SOD1 itself is relevant – for understanding sporadic amyotrophic lateral sclerosis. Although dozens of potential therapies have shown promise in the mice, so far only riluzole has proven to help patients in clinical trials. Those results raised concerns that SOD1 might not be a factor in the majority of amyotrophic lateral sclerosis cases after all.

"The Ohio team has developed a useful model for addressing these issues," said Amelie Gubitz PhD, a program director at NINDS. "Their analysis of the cells adds to mounting evidence that astrocytes – and particularly SOD1 function in those cells – contribute to both sporadic and familial ALS."

The investigators obtained postmortem spinal tissue from amyotrophic lateral sclerosis patients through the National Disease Research Interchange (NDRI). This is a nonprofit organization supported by NIH, voluntary health organizations, and corporate and individual sponsors that enables people to donate their tissues for use in research after death, and then delivers specific tissues to research labs. NDRI performed the autopsies in the amyotrophic lateral sclerosis patients' local communities and used couriers to fly the tissue samples to Columbus. By working through weekends and often overnight, the Columbus team was able to process the samples in the current study within 2 to 3 days of death.

First, the researchers isolated neural progenitor cells from the tissue and coaxed these cells to become astrocytes. Neural progenitor cells are the parent lineage of neurons and astrocytes. Next, the team combined the patient-derived astrocytes with mouse motor neurons. At first, the motor neurons grew normally, but after 4 days, they began to degenerate. By 5 days, the number of motor neurons was reduced by about half, compared to motor neurons that had been grown with control astrocytes. Similar results were seen when the motor neurons were grown with astrocytes from a patient with familial amyotrophic lateral sclerosis, or with a cell culture broth that had been conditioned by astrocytes from any of the amyotrophic lateral sclerosis patients. This suggests the astrocytes are releasing 1 or more unknown substances that are toxic to motor neurons.

The researchers found that inflammatory responses may play a role in this toxicity. They analyzed 84 genes involved in inflammation, and found that 35% to 60% of the genes showed increased activity in amyotrophic lateral sclerosis astrocytes compared to controls.

Further experiments revealed that the SOD1 plays a critical role in the toxicity. The investigators used a method called RNA interference to silence the SOD1 gene. RNA serves as an intermediary between genes and proteins, but in RNA interference, small RNA fragments are used to block a gene from making proteins. When the researchers used a virus to deliver such small RNAs to astrocytes affected by familial amyotrophic lateral sclerosis, the astrocytes were no longer toxic to motor neurons. This method also suppressed toxicity in 4 of 6 astrocyte lines derived from people with sporadic amyotrophic lateral sclerosis, supporting the idea that the SOD1 enzyme also has a role in sporadic cases.

The results also suggest the need for further investigation of SOD1 and astrocytes as targets for therapy. For example, drugs or small molecules, such as the RNA fragments tested in this study, might be used to reduce SOD1 function in astrocytes and suppress their toxicity. Drs Kaspar and Burghes are developing a viral system that would carry these RNA fragments through the bloodstream and into the spinal cord, and deliver them to astrocytes. This work is being done in collaboration with Don Cleveland PhD, at the University of California, San Diego, as part of the Recovery Act grant. Several labs are pursuing cell replacement therapies for amyotrophic lateral sclerosis, and the current work adds to evidence that replacing astrocytes may be just as important as, and perhaps easier than, replacing motor neurons lost to the disease.

"It's been a long road, but the hard work starts now," Dr Kaspar said. "We still need to confront fundamental questions about what is happening to astrocytes and how they are killing motor neurons. And the ultimate goal is to identify therapies that will translate into helping humans."

Additional funding came from Project A.L.S., the Packard Center for ALS Research, the Helping Link Foundation, and the Swiss National Science Foundation.

Source: News Release
NIH/National Institute of Neurological Disorders and Stroke
August 10, 2011